SIMPLE METHODS FOR DETERMINING POTENT XENOBIOTICS IN WATER AND FOODSTUFFS
Main Tasks

TASK 1; Synthesis of immunogen, fluorotracers and antisera to sulfamethoxazole
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To prepare derivatives of the drug

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To covalently link the derivatives from a. to carrier proteins

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To use the immunogen from b. in rabbits to produce antisera

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To prepare fluorescein derivatives from a. for use in polarization fluoroimmunoassay development

TASK 2; Development of polarization fluoroimmunoassays 

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To use the labels derived from Task 1 to screen for the successful generation of antibodies to sufamethoxazole using changes in polarization of fluorescence to monitor antibody binding

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To develop and fully validate a polarization fluoroimmunoassay (PFIA) for sulfamethoxazole

TASK 3; Development and validation of dip strip ELISA assays for steroid estrogens, beta-lactams and sulfamethoxazole

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To transfer technology regarding the preparation of coated dip sticks and ELISA conditions for estrogenic steroids and cephalosporin antibiotics

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To supply antisera for steroid estrogens and cephalosporin antibiotics

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To develop dip stick ELISAs for sulfamethoxazole, estrogenic steroids and sulfamethoxazole.

TASK 4; Development of surface plasmon resonance immunobiosensors

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To immobilise antisera obtained on to optical sensing surfaces

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To develop and validate assays for the three drugs based on surface plasmon resonance.

TASK 5; Development of mutagenicity tests

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To further develop existing electrochemical biosensors based on immobilised DNA and demonstrate their application to the detection of selected mutagens including polyaromatic hydrocarbons, antineoplastic drugs and heterocyclic amines.

TASK 6; Preparation and validation of extraction systems (IES)

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To determine the efficiency of extraction of the resulting IES in column formats for water, bread and milk spiked with the appropriate drug using the assay systems developed in Tasks 2, 3 and 4

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To immobilise the antisera on cellulose nitrate dip sticks

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To use the IES systems with samples of water, milk and bread supplied by each partner and to send extracts for analysis of the three drugs by dip stick/PFIA, SPR and GC-MS, LC-MS methods as well as to screen the extracts for mutagenicity.

 

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